From Gene Engineering to Industrial Production: JINMAI Biotech Achieves Breakthrough in Chitosanase (CSN) Manufacturing

JINMAI Biotech is pleased to announce a significant milestone in its industrial enzyme development program. After extensive research and process optimization, the company’s R&D team has successfully established a scalable production process for Chitosanase (CSN) through microbial strain engineering and fermentation technology.

The achievement represents another important step in JINMAI’s synthetic biology and industrial biotechnology strategy, strengthening the company’s capability in enzyme development, precision fermentation, and green manufacturing.

Accelerating Industrial Enzyme Innovation

Industrial enzymes are becoming increasingly important in agriculture, environmental protection, food processing, and biotechnology. Among them, Chitosanase (EC 3.2.1.132) is attracting growing attention because of its ability to convert high-molecular-weight chitosan into bioactive chitooligosaccharides (COS) under mild reaction conditions.

Compared with conventional chemical hydrolysis, enzymatic conversion offers several advantages:

• Higher substrate specificity

• Mild reaction conditions

• Better product consistency

• Lower environmental impact

• Reduced chemical residues

• Improved control over molecular weight distribution

These advantages make enzymatic production an increasingly attractive approach for manufacturing high-quality chitooligosaccharides.

Understanding Chitosanase (CSN)

Chitosanase is a glycoside hydrolase that specifically hydrolyzes the β-1,4 glycosidic bonds in chitosan.

Unlike acid or alkali hydrolysis, Chitosanase selectively cleaves chitosan polymer chains, producing water-soluble chitooligosaccharides while minimizing undesirable degradation.

Chitooligosaccharides have been widely studied for their biological activities and are being explored in various fields including:

• Agricultural biostimulants

• Plant health management

• Aquaculture

• Cosmetics

• Functional food ingredients

• Biomaterials

In agriculture, chitooligosaccharides have been reported to help activate natural plant defense responses, improve stress tolerance, and promote healthy crop growth.

Overcoming the Challenges of Large-Scale Chitosanase Production

Although Chitosanase has significant application potential, industrial production has traditionally faced several technical challenges, including:

• Low enzyme yield

• Limited enzyme stability

• High manufacturing cost

• Difficulty controlling enzymatic hydrolysis

• Complex downstream purification

To address these challenges, JINMAI’s R&D team carried out systematic optimization covering the complete development process, including:

• Microbial strain engineering

• Expression system optimization

• Precision fermentation

• Fermentation parameter optimization

• Enzyme activity stabilization

• Purification process improvement

• Laboratory application validation

The resulting manufacturing process delivers improved production efficiency, enhanced enzyme stability, and a scalable production pathway suitable for industrial applications.

Building a Complete Enzyme Development Platform

The successful development of industrial-scale Chitosanase production reflects JINMAI’s long-term investment in synthetic biology and fermentation engineering.

The company has established an integrated technology platform covering:

• Gene design

• Vector construction

• Microbial strain development

• Fermentation process optimization

• Enzyme purification

• Quality control

• Pilot-scale verification

• Industrial production

This end-to-end R&D capability enables faster commercialization of enzyme products while maintaining product consistency and manufacturing quality.

Advancing Green Biomanufacturing

As industries continue to seek more sustainable manufacturing technologies, enzyme catalysis offers an environmentally friendly alternative to many conventional chemical processes.

JINMAI believes precision fermentation and enzyme engineering will play an increasingly important role in future agricultural and industrial biotechnology.

The successful industrialization of Chitosanase further expands the company’s enzyme portfolio and supports its long-term strategy of developing high-performance biological solutions through synthetic biology.

Looking Ahead

Building on this achievement, JINMAI will continue to invest in:

• Directed enzyme evolution

• Protein engineering

• Fermentation process optimization

• Cost reduction through biomanufacturing

• Expanded industrial applications

Future research will explore the application potential of Chitosanase in sustainable agriculture, biodegradable materials, environmental biotechnology, and other emerging bio-based industries.

Through continuous innovation, JINMAI remains committed to providing efficient, sustainable, and scientifically advanced enzyme solutions for customers worldwide.

About JINMAI Biotech

JINMAI Biotech specializes in agricultural biotechnology, microbial fermentation, biostimulants, industrial enzymes, and precision fermentation technologies. The company is committed to developing innovative biological products through synthetic biology, microbial engineering, and sustainable manufacturing, delivering environmentally responsible solutions for modern agriculture and biotechnology.